Factors That Affect Human Islet Isolation

Successful human islet isolation utilizing recombinant collagenase.

The enzymatic dissociation of acinar tissue by collagenase is a substantial step in the isolation of pancreatic islets. Although essential collagenase components have been purified, the variability in the activity of different batches limits long-term reproducibility of isolation success. The utilization of purified recombinant proteases would solve this problem. In the present study, pancrease...

Technical challenges in human islet isolation

Ståhle, M. 2015. Technical challenges in human islet isolation. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1119. 57 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-9283-0. Transplantation of islets of Langerhans is an effective treatment option for patients with brittle type 1 diabetes mellitus. This treatment restores glucose control and ...

identifying and prioritizing factors that affect human resource productivity by using madm techniques

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Factors Influencing the Early Results of Pancreatic Islet Transplantation: Studies on Isolation Enzymes and Islet Revascularisation

Murine pancreatic islet isolation.

Name Type Company Catalog Number Comments Dithizone reagent Dissolve 5mg Dithizone in 5 ml of DMSO in a 50 ml conical tube. Add 45 ml of normal saline and mix gently. Filter with a 60 cc syringe and a 0.22 "m syringe filter into a new 50 ml tube. Store at 4 OC, until use. Add 1 ml to each 35 mm counting Grid-plate, when needed. PBS Buffer HBSS Buffer Isolation soln. Buffer HBSS containing 10 mM...